3X0E
Crystal structure of the ectodomain of human CD81 large extracellular loop (hCD81-LEL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.124, 77.408, 38.497 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 29.610 - 1.844 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BKH |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.901 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.625 | 1.940 |
| High resolution limit [Å] | 1.834 | 1.830 |
| Rmerge | 0.016 | 0.515 |
| Number of reflections | 14801 | |
| <I/σ(I)> | 16.63 | 5 |
| Completeness [%] | 97.0 | 86.1 |
| Redundancy | 6.6 | 5.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M Magnesium acetate 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
