3X0E
Crystal structure of the ectodomain of human CD81 large extracellular loop (hCD81-LEL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.124, 77.408, 38.497 |
Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
Resolution | 29.610 - 1.844 |
R-factor | 0.1965 |
Rwork | 0.195 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4BKH |
RMSD bond length | 0.007 |
RMSD bond angle | 0.901 |
Data reduction software | XDS (package) |
Data scaling software | XDS (package) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.625 | 1.940 |
High resolution limit [Å] | 1.834 | 1.830 |
Rmerge | 0.016 | 0.515 |
Number of reflections | 14801 | |
<I/σ(I)> | 16.63 | 5 |
Completeness [%] | 97.0 | 86.1 |
Redundancy | 6.6 | 5.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M Magnesium acetate 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |