3WYC
Structure of a meso-diaminopimelate dehydrogenase in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 123.021, 123.021, 193.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.120 - 2.070 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.070 | 4.460 | 2.070 |
| Rmerge | 0.084 | 0.059 | 0.296 |
| Number of reflections | 66282 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.9 | 98.7 | 100 |
| Redundancy | 5.6 | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | trisodium citrate dihydrate, TES, NADP, D-Lys, pH 7.5, vapor diffusion, sitting drop, temperature 293K |
