3WW0
Crystal structure of F97A mutant, a new nuclear transport receptor of Hsp70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | AREA DETECTOR |
| Collection date | 2013-11-08 |
| Wavelength(s) | 0.9192 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.483, 85.483, 68.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.741 - 2.500 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.735 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 12674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293.15 | 0.1M Tris-HCl (pH 7.4), 1M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
