3WV6
Crystal Structure of a protease-resistant mutant form of human galectin-9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.340, 94.870, 64.810 |
Unit cell angles | 90.00, 113.87, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.850 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.099 | 0.286 |
Number of reflections | 46506 | |
<I/σ(I)> | 13.5 | 3.99 |
Completeness [%] | 98.9 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M L-proline, 4%(w/v) PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |