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3WTU

Crystal structure of the complex comprised of ETS1 (V170A), RUNX1, CBFBETA, and the tcralpha gene enhancer DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyCCD
Collection date2009-04-22
DetectorADSC QUANTUM 315
Wavelength(s)1.0
Spacegroup nameP 21 21 21
Unit cell lengths78.558, 102.110, 194.945
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.740 - 2.700
R-factor0.232
Rwork0.232
R-free0.28200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3wts
RMSD bond length0.009
RMSD bond angle1.300
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.800
High resolution limit [Å]2.7002.700
Rmerge0.0600.321
Number of reflections43481
<I/σ(I)>14.7
Completeness [%]99.294.8
Redundancy11.89.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72984% PEG 8000, 0.1M AMMONIUM ACETATE, 0.02M MAGNESIUM CHLORIDE, 0.05M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

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