3WQB
Crystal structure of aeromonas sobria serine protease (ASP) and the chaperone (ORF2) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 152.062, 152.062, 51.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.740 - 1.410 |
| R-factor | 0.1877 |
| Rwork | 0.187 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hjr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 3.830 | 1.410 |
| Rmerge | 0.088 | 0.065 | 0.381 |
| Number of reflections | 130476 | ||
| <I/σ(I)> | 21 | ||
| Completeness [%] | 99.2 | 93.9 | 97.9 |
| Redundancy | 6.3 | 7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 7.5% PEG 3000, 0.05M HEPES, 0.05M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
