3WPT
Crystal structure of closed dimer of human importin-alpha1 (Rch1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 139.299, 139.299, 141.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.505 - 2.629 |
R-factor | 0.191 |
Rwork | 0.190 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jdq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.781 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8.1_1168) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.510 | 2.723 |
High resolution limit [Å] | 2.629 | 2.629 |
Rmerge | 0.065 | 0.391 |
Number of reflections | 40635 | |
<I/σ(I)> | 31.83 | 4.86 |
Completeness [%] | 97.0 | 90.25 |
Redundancy | 8.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 50MM MES, 100MM AMMONIUM SULFATE, 10MM MGCL, 15-20%(w/v) PEG8000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |