3WPT

Crystal structure of closed dimer of human importin-alpha1 (Rch1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32B2
Synchrotron site	SPring-8
Beamline	BL32B2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	139.299, 139.299, 141.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.505 - 2.629
R-factor	0.191
Rwork	0.190
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jdq
RMSD bond length	0.003
RMSD bond angle	0.781
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.1_1168)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.723
High resolution limit [Å]	2.629	2.629
Rmerge	0.065	0.391
Number of reflections	40635
<I/σ(I)>	31.83	4.86
Completeness [%]	97.0	90.25
Redundancy	8.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	50MM MES, 100MM AMMONIUM SULFATE, 10MM MGCL, 15-20%(w/v) PEG8000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K