3WPC
Crystal structure of horse TLR9 in complex with agonistic DNA1668_12mer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-21 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 68.762, 81.624, 81.519 |
Unit cell angles | 87.50, 83.50, 85.98 |
Refinement procedure
Resolution | 47.490 - 1.600 |
R-factor | 0.18575 |
Rwork | 0.184 |
R-free | 0.22022 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.683 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 211265 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 1% PEG 4000, 1% 2-propanol, 0.2M Na acetate, 0.1M Na citrate pH5.8, 5% DMSO, 1/10 volume of Silver Bullet A6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |