3WNW

Structure of Mouse H-chain modified ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.947
Spacegroup name	P 42 21 2
Unit cell lengths	218.145, 218.145, 147.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.860 - 2.240
R-factor	0.2227
Rwork	0.220
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ies
RMSD bond length	0.020
RMSD bond angle	1.911
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.240	6.100	2.250
Rmerge	0.143	0.054	0.561
Number of reflections	161908
<I/σ(I)>	5.8
Completeness [%]	95.6	99.9	95.5
Redundancy	4.7	8.1	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	286	0.1M Na-cacodylate PH 6.5, 0.2 M Mg-Ac, 30% MPD, vapor diffusion, sitting drop, temperature 286K