3WNU
The crystal structure of catalase-peroxidase, KatG, from Synechococcus PCC7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.744, 108.744, 202.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.390 - 2.200 |
| R-factor | 0.16956 |
| Rwork | 0.168 |
| R-free | 0.20602 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.950 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.155 | |
| Number of reflections | 63054 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.6 | 95.9 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | Sodium formate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
