3WL2
Monoclinic Lysozyme at 0.96 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-06 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.353, 62.563, 60.090 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 30.045 - 0.960 |
| R-factor | 0.16138 |
| Rwork | 0.160 |
| R-free | 0.19532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.914 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP (11.1.03) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.045 | |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.054 | |
| Number of reflections | 111914 | |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 298 | 50mM Sodium Acetate pH4.7, 20% Ethylene glycol, 0.4M Sodium nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
