3WHO
X-ray-Crystallographic Structure of an RNase Po1 Exhibiting Anti-tumor Activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-27 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 31 |
Unit cell lengths | 75.557, 75.557, 34.803 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.730 - 1.850 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bu4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.780 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.157 | 0.627 |
Number of reflections | 18553 | 1561 |
<I/σ(I)> | 10.1 | 2.5 |
Completeness [%] | 99.6 | 84.7 |
Redundancy | 7.7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4M sodium formate, 0.1M Bis-TRIS propane, 10% PEG400, 0.75M cesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |