3WGK
STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAG, DeltaT7GAG-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.335, 95.927, 72.136 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 37.446 - 2.799 |
| R-factor | 0.2036 |
| Rwork | 0.200 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.799 | 6.030 | 2.800 |
| Rmerge | 0.093 | 0.082 | 0.430 |
| Number of reflections | 14645 | ||
| Completeness [%] | 99.7 | 96.9 | 99.7 |
| Redundancy | 3.8 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium Acetate, 0.1M Tris-HCl, 30%(w/v) PEG4000, vapor diffusion, sitting drop, temperature 293K |
