3WC2

Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with a tRNA(Phe)(GUG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 31 2 1
Unit cell lengths	96.740, 96.740, 299.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.090 - 3.641
R-factor	0.2552
Rwork	0.253
R-free	0.28750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbz
RMSD bond length	0.004
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.711		3.860
High resolution limit [Å]	3.640	10.690	3.640
Rmerge	0.107	0.032	1.200
Rmeas	0.113	0.034	1.180
Number of reflections	18933	827	2949
<I/σ(I)>	16.68	60.02	2.05
Completeness [%]	99.6	97.1	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	Bis-Tris propan, CaCl2, PEG 3350, pH 6.0, vapor diffusion, sitting drop, temperature 293K