3WC0
Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.430, 217.560, 140.910 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 44.580 - 3.030 |
| R-factor | 0.2012 |
| Rwork | 0.198 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wbz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.580 | 3.030 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.738 | |
| Number of reflections | 98555 | |
| <I/σ(I)> | 2.03 | |
| Completeness [%] | 98.8 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Bis-Tris propan, NaNO3, PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
