3WBF
Crystal Structure of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum co-crystallized with NADP+ and DAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.351, 99.687, 67.172 |
Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.120 |
Rwork | 0.198 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bio |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 30.000 | 2.200 |
High resolution limit [Å] | 2.120 | 4.560 | 2.120 |
Rmerge | 0.099 | 0.057 | 0.325 |
Number of reflections | 61060 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 99.2 | 99.4 | 93.9 |
Redundancy | 3.6 | 3.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | MPD, PEG 6000, NADP+, DAP,, pH 7.5, vapor diffusion, sitting drop, temperature 288K |