3WBF
Crystal Structure of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum co-crystallized with NADP+ and DAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.351, 99.687, 67.172 |
| Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.120 |
| Rwork | 0.198 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bio |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 30.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 4.560 | 2.120 |
| Rmerge | 0.099 | 0.057 | 0.325 |
| Number of reflections | 61060 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.2 | 99.4 | 93.9 |
| Redundancy | 3.6 | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | MPD, PEG 6000, NADP+, DAP,, pH 7.5, vapor diffusion, sitting drop, temperature 288K |
