3WAJ
Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) complex with Zn and sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.195, 108.961, 56.069 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 38.813 - 2.501 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wai |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.813 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.049 | 0.512 |
| Number of reflections | 47863 | |
| <I/σ(I)> | 22.9 | 2.3 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M zinc sulfate, 0.1M sodium acetate buffer, 15% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
