3W9W
Crystal structure of DING protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.270, 87.140, 88.600 |
| Unit cell angles | 90.00, 90.82, 90.00 |
Refinement procedure
| Resolution | 43.570 - 1.350 |
| R-factor | 0.1495 |
| Rwork | 0.148 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.570 | 1.430 | |
| High resolution limit [Å] | 1.350 | 4.040 | 1.350 |
| Rmerge | 0.058 | 0.026 | 0.385 |
| Rmeas | 0.031 | 0.456 | |
| Number of reflections | 150208 | 5947 | 21622 |
| <I/σ(I)> | 16.15 | 44.86 | 3.21 |
| Completeness [%] | 95.9 | 99.2 | 85.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 8 | 298 | Tris, PEG 8000, NaCl, sitting drop, temperature 298K |
