3W8H

Crystal structure of CCM3 in complex with the C-terminal regulatory domain of STK25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2011-07-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	68.560, 68.560, 229.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.238 - 2.426
R-factor	0.2118
Rwork	0.209
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ajm
RMSD bond length	0.003
RMSD bond angle	0.684
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.340	2.560
High resolution limit [Å]	2.426	2.430
Rmerge	0.106	0.323
Number of reflections	12822
<I/σ(I)>	21.4	7.7
Completeness [%]	98.9	95.8
Redundancy	12.6	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Bis-Tris, 25% PEG3350, 0.2M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K