3W81

Human alpha-l-iduronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	H 3
Unit cell lengths	259.225, 259.225, 71.835
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.560 - 2.300
R-factor	0.1711
Rwork	0.169
R-free	0.20990
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.370
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	0.468
Number of reflections	78102
<I/σ(I)>	13.2	3
Completeness [%]	97.4	80.6
Redundancy	6.6	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	18%(w/v) PEG 3350, 0.18M K/Na tartrate, 3%(w/v) PEG MME 5000, 0.02M ammonium sulfate, 0.01M MES-Na, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	18%(w/v) PEG 3350, 0.18M K/Na tartrate, 3%(w/v) PEG MME 5000, 0.02M ammonium sulfate, 0.01M MES-Na, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K