3W5H
Ultra-high resolution structure of NADH-cytochrome b5 reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 40 |
| Detector technology | CCD |
| Collection date | 2011-02-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.480, 72.108, 84.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.780 |
| R-factor | 0.1268 |
| Rwork | 0.126 |
| R-free | 0.14390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INDH |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.790 |
| High resolution limit [Å] | 0.780 | 0.780 |
| Rmerge | 0.052 | 0.350 |
| Number of reflections | 314321 | |
| <I/σ(I)> | 26.1 | 2 |
| Completeness [%] | 94.5 | 70.5 |
| Redundancy | 4.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 4000, 10mM potassium phosphate, 5mM dithionite, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
