3W3Z

Crystal structure of Kap121p bound to RanGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-14
Detector	RAYONIX MX225HE
Wavelength(s)	0.97897
Spacegroup name	P 32 2 1
Unit cell lengths	97.750, 97.750, 289.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.360 - 2.700
R-factor	0.2624
Rwork	0.261
R-free	0.29778
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.627
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	45100
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES, 0.2M CaCl2, 10% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K