3W2Y
Crystal structure of DNA uridine endonuclease Mth212 mutant W205S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-12-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 |
Unit cell lengths | 100.295, 100.295, 144.698 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.173 |
Rwork | 0.172 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fzi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.133 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.091 | 0.060 | 0.450 |
Number of reflections | 64714 | ||
<I/σ(I)> | 10.8 | 33.2 | 6.4 |
Completeness [%] | 99.8 | 97.7 | 100 |
Redundancy | 12.2 | 11.1 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12.5% PEG3350, 50mM Magnesium formate dihydrate, 11.5mM uracil, VAPOR DIFFUSION, HANGING DROP, temperature 293K |