3W1B
Crystal Structure of Human DNA ligase IV-Artemis Complex (Mercury Derivative)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0036, 1.0093, 0.9915 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 68.630, 105.210, 122.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.820 - 2.400 |
| R-factor | 0.1791 |
| Rwork | 0.176 |
| R-free | 0.22530 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.820 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.536 |
| Number of reflections | 34931 | |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 2M ammonium sulfate, 10mM YCl, 100mM MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
