3VZ0
Structural insights into cofactor and substrate selection by Gox0499
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791, 0.9793, 0.96 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.523, 254.602, 76.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.490 - 2.300 |
R-factor | 0.18943 |
Rwork | 0.187 |
R-free | 0.24410 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.319 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 77510 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 500, CaCl2, Bis-Tris, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |