3VXV
Crystal structure of methyl CpG Binding Domain of MBD4 in complex with the 5mCG/TG sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.074, 94.989, 54.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.747 - 2.000 |
| R-factor | 0.1892 |
| Rwork | 0.188 |
| R-free | 0.22350 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.031 | 0.365 |
| Number of reflections | 16062 | |
| <I/σ(I)> | 18.2 | 4.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 2 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
