3VXT
T36-5 TCR specific for HLA-A24-Nef134-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.768, 65.036, 92.275 |
| Unit cell angles | 91.30, 94.19, 105.93 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.230 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kxf |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.028 | 0.222 | |
| Number of reflections | 32447 | ||
| <I/σ(I)> | 23.5 | ||
| Completeness [%] | 91.9 | 93.7 | 81.6 |
| Redundancy | 3 | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M potassium sulfate, 0.1M MES, 15% PEG8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
