3VWU

Crystal structure of peroxiredoxin 4 from M. musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-15
Detector	RAYONIX MX225HE
Wavelength(s)	0.90
Spacegroup name	P 21 2 21
Unit cell lengths	87.468, 118.656, 255.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.108 - 3.300
R-factor	0.2054
Rwork	0.203
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	3.380
High resolution limit [Å]	3.300	3.300
Rmerge	0.104
Number of reflections	38676
<I/σ(I)>	13.9
Completeness [%]	94.6	88.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	8-10% PEG-MME 550, 50mM MES, pH 6.5, VAPOR DIFFUSION, temperature 293K