3VRQ

Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2008-06-18
Detector	Bruker DIP-6040
Wavelength(s)	0.900
Spacegroup name	P 1 21 1
Unit cell lengths	62.600, 66.211, 81.558
Unit cell angles	90.00, 106.02, 90.00

Refinement procedure

Resolution	37.980 - 2.390
R-factor	0.2176
Rwork	0.215
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.468
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.390	5.170	2.400
Rmerge	0.062	0.023	0.373
Number of reflections	25297
<I/σ(I)>	22.777	46.682	4.006
Completeness [%]	100.0	99.8	100
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	22% PEG3350, 0.1M Magnesium Nitrate, 0.6M NDSB-201, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K