3VR8

Mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2004-10-05
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	123.707, 129.090, 221.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 2.810
R-factor	0.23446
Rwork	0.231
R-free	0.29717
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zoy
RMSD bond length	0.014
RMSD bond angle	1.699
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.117	0.517
Number of reflections	79622
<I/σ(I)>	7.9	1.26
Completeness [%]	92.5	67.5
Redundancy	8.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	8.4	293	15% (w/v) PEG 3350, 100mM Tris-HCl pH 8.4, 200mM NaCl, 1mM sodium malonate, 0.06% (w/v) C12E8, 0.04% (w/v) C12M, MICRODIALYSIS, temperature 293K