3VQ2
Crystal structure of mouse TLR4/MD-2/LPS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.813, 144.320, 86.940 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 86.590 - 2.480 |
| R-factor | 0.22302 |
| Rwork | 0.221 |
| R-free | 0.26728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z64 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 86.600 |
| High resolution limit [Å] | 2.480 |
| Number of reflections | 70034 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 18% PEG3350, 0.2M sodium tartrate, 0.1M Bis-Tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
