3VPC
ArgX from Sulfolobus tokodaii complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.266, 67.845, 80.032 |
| Unit cell angles | 87.77, 75.29, 67.03 |
Refinement procedure
| Resolution | 34.960 - 1.870 |
| R-factor | 0.20078 |
| Rwork | 0.198 |
| R-free | 0.25314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uc8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 98303 | |
| Completeness [%] | 94.4 | 87.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M (NH4)H2PO4, 10% PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
