3VOM
Structure of a putative phosphoserine aminotransferase from mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.320, 94.340, 101.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.790 - 2.100 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 4.490 | 2.100 |
| Rmerge | 0.121 | 0.066 | 0.365 |
| Number of reflections | 42955 | ||
| <I/σ(I)> | 7.8 | 3.4 | |
| Completeness [%] | 98.4 | 94.1 | 99.3 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | Tris-HCl 0.1M, NH4SO4 2.2M, pH 8.5, vapor diffusion, sitting drop, temperature 291K |
