3VKG

X-ray structure of an MTBD truncation mutant of dynein motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Collection date	2011-04-25
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	204.256, 221.806, 192.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.450 - 2.810
R-factor	0.262
Rwork	0.262
R-free	0.31900
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	150.000
High resolution limit [Å]	2.000
Rmerge	0.092
Number of reflections	209488
<I/σ(I)>	17.6
Completeness [%]	98.3
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	3.0-6.0% PEG 8000, 10MM SPERMINE-HCL, 3MM ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K