3VK7
Crystal structure of DNA-glycosylase bound to DNA containing 5-Hydroxyuracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.703, 121.594, 81.121 |
| Unit cell angles | 90.00, 95.49, 90.00 |
Refinement procedure
| Resolution | 28.296 - 2.100 |
| R-factor | 0.2179 |
| Rwork | 0.213 |
| R-free | 0.26620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a46 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.080 | 0.048 | 0.232 |
| Number of reflections | 41763 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 92.6 | 97.6 | 98.9 |
| Redundancy | 2.8 | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 285 | PEG 3350, HCOONa, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
