3VJB
Crystal structure of the human squalene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.610, 153.860, 91.516 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.050 |
| R-factor | 0.17164 |
| Rwork | 0.169 |
| R-free | 0.22729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ezf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.077 | 0.402 |
| Number of reflections | 144874 | |
| <I/σ(I)> | 20.8 | 3.6 |
| Completeness [%] | 98.5 | 91.4 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M sodium citrate tribasic dihydrate, 0.1M HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
