3VJ9
Crystal structure of the human squalene synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.776, 76.657, 82.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.700 - 1.520 |
R-factor | 0.16284 |
Rwork | 0.160 |
R-free | 0.21376 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ezf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.419 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.045 | 0.509 |
Number of reflections | 51144 | |
<I/σ(I)> | 40.9 | 4.1 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 8 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PEG 2000 MME, 0.01M NiCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |