3VI3
Crystal structure of alpha5beta1 integrin headpiece (ligand-free form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-04-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 |
Unit cell lengths | 92.956, 102.805, 125.079 |
Unit cell angles | 76.10, 70.19, 71.28 |
Refinement procedure
Resolution | 100.000 - 2.900 |
R-factor | 0.21001 |
Rwork | 0.207 |
R-free | 0.26683 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.262 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.390 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 90446 | |
<I/σ(I)> | 11.5 | 2.1 |
Completeness [%] | 98.8 | 98.3 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-tris (pH6.5), 20%(w/v) PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |