3VH3

Crystal structure of Atg7CTD-Atg8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2011-01-24
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	69.815, 69.815, 220.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.280 - 2.000
R-factor	0.232
Rwork	0.232
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zpn 3vh1
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.076	0.475
Number of reflections	37933
<I/σ(I)>	53.7	4.2
Completeness [%]	99.7	99.4
Redundancy	9.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% PEGMME 5000, 5% tacsimate, 0.1M HEPES , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K