3VH2
Crystal structure of Saccharomyces cerevisiae Atg7 (1-613)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-07-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.028, 88.028, 264.249 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.040 - 3.300 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vh1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.151 | 0.480 |
| Number of reflections | 18578 | |
| <I/σ(I)> | 27.5 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 9-13% ethylene glycol, 150mM NaCl, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
