3VH1
Crystal structure of Saccharomyces cerevisiae Atg7 (1-595)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.27 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.327, 88.327, 264.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25000 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.070 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | 0.437 |
Number of reflections | 24834 | |
<I/σ(I)> | 50.3 | 4.1 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 20.7 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 9-13% ethylene glycol, 150mM NaCl, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |