3VGJ
Crystal of Plasmodium falciparum tyrosyl-tRNA synthetase (PfTyrRS)in complex with adenylate analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-12-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.560, 46.500, 141.350 |
Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
Resolution | 40.025 - 2.212 |
R-factor | 0.1739 |
Rwork | 0.172 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
High resolution limit [Å] | 2.210 | 4.760 | 2.210 |
Rmerge | 0.056 | 0.039 | 0.405 |
Number of reflections | 43059 | ||
<I/σ(I)> | 17.6 | ||
Completeness [%] | 94.7 | 97.9 | 82.2 |
Redundancy | 14.1 | 14 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 10mM MgCl2, 10mM ATP, 2mM L-Tyrosine, 2.4mM sodium malonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |