3VEK
Both Zn Fingers of GATA1 Bound to Palindromic DNA Recognition Site, P1 Crystal Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 35.195, 62.415, 66.046 |
Unit cell angles | 72.39, 74.57, 73.63 |
Refinement procedure
Resolution | 48.680 - 2.630 |
R-factor | 0.2369 |
Rwork | 0.235 |
R-free | 0.27510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vd6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.921 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.680 |
High resolution limit [Å] | 2.630 | 7.130 | 2.630 |
Rmerge | 0.088 | 0.036 | 0.373 |
Number of reflections | 14526 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 97.0 | 98.1 | 94.3 |
Redundancy | 4.7 | 4.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 6000, 0.01M ZnCl2, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |