3VE0
Crystal structure of Sudan Ebolavirus Glycoprotein (strain Boniface) bound to 16F6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | I 2 3 |
Unit cell lengths | 194.859, 194.859, 194.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.930 - 3.353 |
R-factor | 0.22523 |
Rwork | 0.222 |
R-free | 0.28949 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3csy |
RMSD bond length | 0.012 |
RMSD bond angle | 1.538 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.049 | 0.691 |
Number of reflections | 17818 | |
<I/σ(I)> | 13.4 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.4 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 300 | 15% (w/v) PEG 3350, 0.2 M lithium citrate, and 1.5% benzamidine hydrochloride, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |