3VCL

Crystal Structure of HLA-B7 with the HCMV pp65 peptide RPHERNGFTVL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Collection date	2009-07-27
Spacegroup name	P 41 21 2
Unit cell lengths	65.786, 65.786, 237.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.281 - 1.700
R-factor	0.1859
Rwork	0.184
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h6p
RMSD bond length	0.018
RMSD bond angle	1.699
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.281
High resolution limit [Å]	1.700
Rmerge	0.055
Number of reflections	56534
<I/σ(I)>	37
Completeness [%]	99.2
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	18% PEG2000-MME, 5-10mM NiCl2, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K