3VCL
Crystal Structure of HLA-B7 with the HCMV pp65 peptide RPHERNGFTVL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Collection date | 2009-07-27 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.786, 65.786, 237.107 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.281 - 1.700 |
R-factor | 0.1859 |
Rwork | 0.184 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h6p |
RMSD bond length | 0.018 |
RMSD bond angle | 1.699 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.281 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.055 |
Number of reflections | 56534 |
<I/σ(I)> | 37 |
Completeness [%] | 99.2 |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 18% PEG2000-MME, 5-10mM NiCl2, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |