3VAF
Structure of U2AF65 variant with BrU3 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.960 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.296, 36.981, 99.431 |
| Unit cell angles | 90.00, 125.67, 90.00 |
Refinement procedure
| Resolution | 20.510 - 2.490 |
| R-factor | 0.2361 |
| Rwork | 0.231 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g4b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.580 |
| High resolution limit [Å] | 2.490 | 5.360 | 2.490 |
| Rmerge | 0.134 | 0.076 | 0.455 |
| Number of reflections | 17441 | ||
| <I/σ(I)> | 18.3 | ||
| Completeness [%] | 99.3 | 98.1 | 99.3 |
| Redundancy | 6.6 | 6.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
