3VA1
Crystal structure of the mammalian MDC1 FHA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-31 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.076, 48.590, 134.182 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.740 |
| R-factor | 0.19273 |
| Rwork | 0.189 |
| R-free | 0.27176 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.055 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.090 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.750 |
| Rmerge | 0.072 | 0.498 |
| Number of reflections | 25045 | |
| <I/σ(I)> | 38.9 | 2.9 |
| Completeness [%] | 98.4 | 96 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.26M (NH4)2SO4, 0.2M Li2SO4, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
