3V8I
Crystal Structure of a Drosophila melanogaster Dopamine N-Acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.838, 52.511, 67.594 |
| Unit cell angles | 74.05, 90.05, 73.07 |
Refinement procedure
| Resolution | 26.352 - 1.802 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3te4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.997 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.065 | 0.039 | 0.316 |
| Number of reflections | 44004 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 95.2 | 96.9 | 94.6 |
| Redundancy | 4.1 | 3.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 288 | 1.0M NaH2PO4/1.6M K2HPO4, 0.1M HEPES, 0.2M NaCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
