3V8G

Crystal structure of an asymmetric trimer of a glutamate transporter homologue (GltPh)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	203.692, 119.627, 223.415
Unit cell angles	90.00, 113.77, 90.00

Refinement procedure

Resolution	15.000 - 4.660
R-factor	0.25731
Rwork	0.255
R-free	0.29374
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.135
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	89.000
High resolution limit [Å]	4.650
Number of reflections	37155
Completeness [%]	72.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	20 - 25% PEG 350 MME, 0.1 M MES, pH 6.5, 250-450 mM CaCl2, 5% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K