3V7R
Crystal structure of Staphylococcus aureus biotin protein ligase in complex with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 277 |
Collection date | 2009-09-21 |
Wavelength(s) | 1.54 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 94.600, 94.600, 130.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 2.610 |
R-factor | 0.20286 |
Rwork | 0.200 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rkw |
RMSD bond length | 0.022 |
RMSD bond angle | 1.977 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.850 |
High resolution limit [Å] | 2.610 |
Number of reflections | 18442 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |