3V7R
Crystal structure of Staphylococcus aureus biotin protein ligase in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 277 |
| Collection date | 2009-09-21 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 94.600, 94.600, 130.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.610 |
| R-factor | 0.20286 |
| Rwork | 0.200 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rkw |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.977 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.850 |
| High resolution limit [Å] | 2.610 |
| Number of reflections | 18442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
